Activating QIIME2 & Importing Metadata

The point at which the workshop transitions from concepts to commands. Activate the QIIME2 module on Alpine, set up your working directory, copy the workshop metadata, and verify QIIME2 can read it via qiime metadata tabulate.

Activate the QIIME2 Module

module purge
module load qiime2/2026.1_amplicon

Verify the install:

qiime --version

Set Up Your Working Directory

mkdir -p /scratch/alpine/$USER@colostate.edu/qiime2_tutorial
cd /scratch/alpine/$USER@colostate.edu/qiime2_tutorial

Copy the Workshop Metadata

cp /pl/active/courses/2026_summer/CSU_2026/q2_workshop_final/QIIME2/metadata_q2_workshop.txt .

Validate the Metadata

qiime metadata tabulate \
  --m-input-file metadata_q2_workshop.txt \
  --o-visualization metadata_q2_workshop.qzv

Open metadata_q2_workshop.qzv on view.qiime2.org and confirm the columns and types render the way you expect from the metadata decomposition tutorial.

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Next: Importing Sequences